Tampa Bay Vipers Depth Chart
| OFFENSE | |||||
| WR | 17 Jalen Tolliver | 19 Tanner McEvoy | 15 Seantavius Jones | ||
| TE | 85 Nick Truesdell | 80 DeAndre Goolsby | 86 Colin Thompson | 89 Pharoah McKever | |
| LT | 73 Martez Ivey | 78 Marquis Lucas | |||
| LG | 67 Jerald Foster | 78 Marquis Lucas | |||
| C | 63 Jordan McCray | 50 John Yarbrough | |||
| RG | 76 Daronte Bouldin | 50 John Yarbrough | |||
| RT | 71 Isaiah Williams | 69 Na'Ty Rodgers | |||
| WR | 18 Reece Horn | 12 Ryan Davis | |||
| WR | 81 Dan Williams | 1 S.J. Green | |||
| QB | 11 Aaron Murray | 4 Taylor Cornelius | 9 Quinton Flowers | 8 Chase Litton | |
| RB | 24 De'Veon Smith | 29 Jacques Patrick | |||
| DEFENSE | |||||
| DE | 58 Obum Gwacham | 96 Jason Neill | 95 CeCe Jefferson | ||
| DT | 92 Josh Banks | 94 Shane Bowman | |||
| DT | 49 Nikita Whitlock | 97 Ricky Walker | |||
| DE | 53 Deiontrez Mount | 91 Bobby Richardson | |||
| MLB | 45 Lucas Wacha | 43 Emmanuel Beal | |||
| WLB | 41 Terrance Plummer | 52 Reggie Northrup | |||
| CB | 20 Tarvarus McFadden | 31 Trey Caldwell | 32 Jalen Collins | ||
| CB | 23 Anthoula Kelly | 26 Herb Waters | |||
| NCS | 27 Micah Hannemann | 37 Robenson Therezie | |||
| SS | 21 Robert Priester | 30 Demontre Hurst | |||
| FS | 35 Marcelis Branch | 28 Bryce Canady | |||
| SPECIALISTS | |||||
| P | 10 Jake Schum | ||||
| PK | 3 Andrew Franks | ||||
| KO | 3 Andrew Franks | 10 Jake Schum | |||
| H | 10 Jake Schum | 18 Reece Horn | |||
| LS | 42 Nick Moore | 86 Colin Thompson | |||
| PR | 12 Ryan Davis | 18 Reece Horn | |||
| KR | 12 Ryan Davis | ||||